THE EXPIRATION TIME OF STARTER COMPOSED OF INDIGENOUS BACTERIAL AND FUNGI CONSORTIUM ISOLATED FROM FERMENTATION FEED OF SELLULOSIC MATERIALS IN ROOM AND COLD TEMPERATURE

Abstract

The aim of this study determined the storability of the starter which was composed of bacteria and fungi consortium isolated from fermentation feed of cellulosic waste at room and cold temperatures. Cellulosic waste were composed of water hyacinth (Eichhornia crassipes) and corn cobs (Zea mays) which were undergoing a fermentation process was isolated these bacteria and fungi. Furthermore, the bacteria and fungi were purified and given a symbol. Each isolate was tested for cellulolytic activity on CMC media. Isolates with large cellulolytic activity were selected as starter candidates. These candidates were tested for antagonism / synergism work each other. The isolates that were consorted to be starter were isolates with the largest cellulolytic activity and work synergistically. The starter expiration time was determined by the calculate the total number of microorganisms (TPC = total plate count) periodically. Furthermore, the calculation results was matched with the SNI standard for the number of microorganisms for starter (108 cfu / ml). The results of this research was obtained that the total microorganism content of each formula until storage for 65 days at room temperature at 10-13 level dilutions was 147,21 cfu/ml and at cold temperatures 181,37 cfu/ml. The conclusion that can be stated was that the starter is still active at room temperature storage and cold for 65 days. The expiration time of the starter at cold temperatures is longer than the room temperature.