Lipase Immobilization Based On Biopolymer
Lipase (EC 22.214.171.124) or also known as glycerol ester hydrolases are a class of enzyme to break down the hydrolysis of triglycerides into diglycerides, monoglycerides, free fatty acids, and glycerol. The usage of the biopolymer-based carrier in enzyme immobilization has advantages in terms of cheap, being obtained easily and practical. The purpose of this study is using natural polymers such as tapioca starch, gelatin, sodium alginate, and chitosan as lipase immobilization carrier which further analyzed in enzymatic activity assay, enzyme stability, enzyme storage and polymer functional group analysis using FTIR. The natural polymer could be used for lipase immobilization as a result of this study. Immobilized lipase with aminated tapioca starch biopolymer has the highest activity among all biopolymer which is 1.308,7 U/ml with the protein content of 0,207 mg/ml. FTIR result showed a bond formation on (N-H), (CN), (C-H) group in immobilized lipase. Enzyme recovery and immobilized lipase storagetesting with aminated tapioca starch resulted in the highest relative activity on 96,4% and 83,7%.
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