The Difference of Hemoglobin Examination Results in Normal and Hemolysis Samples Using Cyanmethemoglobin Methods
Keywords:Hemolysis, Hemoglobin, Cyanmethemoglobin
Hemoglobin examination is one of the most routine blood tests performed by each laboratory. Blood lysis is mostly caused by the breakdown of diplomatic blood cells. Erythrocyte lysis conditions silenced 15 minutes also can provide an effect on the cell membrane fragility. This study aims to analyze differences in hemoglobin examination results in normal samples and hemolysis using the Cyanmethemoglobin method. This type of research is an experiment. The study population was students and female students of Nahdlatul Ulama University in Surabaya with a sample size of 30 people taken by purposive sampling. Data retrieval is done through the provision of questionnaires and blood tests then analyzed using the One Way ANOVA test, α=0.05. The results showed that of the 30 D-IV Students of Medical Laboratory Technology had an average value of Hb in normal erythrocytes as a control ie 13.1 g/dl, the average Hb level with treatment of lysis erythrocytes without incubation was 13.8 g/dl, the average Hb level with the treatment of lysis erythrocytes with 15 minutes incubation is 12.8 g/dl and the average hemolysis is 1.2%. The results of the analysis through the One Way ANOVA test show that ρ=0.097 which means ρ>α, so that H0 is accepted. So it can be concluded that there is no difference in the results of hemoglobin examination between normal samples and hemolysis in the blood to the routine examination used.
Sukorini. Clinical Laboratory Quality Internal Strengthening. Yogyakarta: Kanal Media dan
Alfa Media. 2010.
Yaqin, M. A.., Arista D. Pre-Analytical Examination Phase Analysis For Improving Quality of
Laboratory Results in RS. Muji Rahayu Surabaya. Science Journal. 2015; 5(10) : p. 1-7.
Dasgupta, A., Sepulveda, J. L. Accurate Results in the Clinical Laboratory: 1 st Editio.
United States: Elsevier. 2013.
Faruq, Z. H. Lysis Blood Analysis of Platelet Value Using the Electrical Impedance Method.
Laboran Medika Journal. 2018; 2(1): p. 11-13.
Hardisari, R., Koiriyah B. Description of Triglyceride Levels (Gpo-Pap Method) in Serum
Samples and EDTA Plasma. Laboratory Technology Journal. 2016: p. 27-31.
Norsiah, W. Differences in Hemoglobin Levels Using the Sianmethemoglobin Method and
Without Centrifugation in Leukocytosis Samples. Medical Laboratory Technology Journal.
: p. 72-83.
Hidayat, N. S. Validity Inspection Method Using Hb Hemoglobin Meter In Young Women In
Man Wonosari. Public health, ISSN: 1978-0575. Yogyakarta: Ahmad Dahlan University.
; 9(1) : p. 11-18.
Dewi, D. C., Durachim, A. Analysis Of Blood Sample Lysis Rate On Hemoglobin
Examination Results Using Rayto Rt. 7600 Auto Hematology Analyzer. Folia Medica
Indonesiana. 2014: p. 262-264.
Giavarina, D., Lippi, G. Blood venous sample collection: Recommendations overview and a
checklist to improve quality, Clinical Biochemistry. The Canadian Society of Clinical
Chemists. 2017; 50(10–11): p. 605–611.
Amrullah, F. H. The effect of giving virgin coconut oil to hemolysis. Science and applied
chemistry. 2009; 3(2): p. 154-163.
Federer, W. T. Experimental Design Theory And Application. 3rd ed: Oxford and IBH
Publishing Co. new Delhi Bombay Calcuta. 1977.
Nugraha, G. Basic Hematology Laboratory Examination Guide. Cv. Trans Info Media.
Istiany, A., Rusilanti. Applied Nutrition. PT. Teenager Rosdakarya. Bandung. 2013.
Siswanto., Sulabda, I. N., Soma, I. G. Fragility of Bali Red Cow Blood Cells. Veterinary
Journal. ISSN : 1411-8327. Bali: Udayana Denpasar University. 2014; 15(1): p. 64-67.
Sowemimo-Coker. Red blood cell hemolysis during processing. NCBI. 2002: p. 46-60.
Ariyadi, T., Sukeksi, A. Difference in Results of Examination of Hemoglobin Levels Direct
and Indirect Cyanmeth Method. 2016. [Online]from: http://Jurnal.unimus.ac.isl.
Fitria, I. P. Relationship between Protein and Iron Substances with Hemoglobin Levels in Patients with Chronic Kidney Failure Post Hemodialysis in Rsud Hemodialysis Unit
Sukoharjo Regency. Surakarta: Publication manuscript. Faculty of Health Sciences, University
of Muhammadiyah. 2016.
How to Cite
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.