The Effect of Moringa oleifera Leaf Extract on Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance


  • L P Wulandari
  • A Hapsari


Moringa oleifera, Theca Cell Thickness, Polycystic Ovary Syndrome


Polycystic ovary syndrome (PCOS) is known as the commonest endocrine disorder in women of reproductive age and often associated with insulin resistance, hyperandrogenemia, chronic inflammation, and oxidative stress. The use of Moringa oleifera as an antioxidant should be investigated as an alternative treatment of follicular refinement in PCOS with insulin resistance. We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the theca cell thickness of PCOS female rat with insulin resistance. This study was a laboratory experimental research. Three month old Rattus norvegicus stran Wistar rat weighing 100- 130 grams were divided into 5 groups (n = 8). PCOS model obtained by giving injection of testosterone propionate for 28 days, followed by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/KgBW for 14 days. Then, we analyzed the thickness of theca cell. Statistical analysis used One Way Anova that was performed with IBM SPSS Statistics versions 24.00. The examination of ovarium histology showed that leaf extract Moringa oleifera 500 mg/KgBW (0.931±0.457) significantly decreased the thickness of theca cells (p <0.05) compared to the PCOS control group. The conclusions was Moringa oleifera leaf extract as an antioxidant proven to decrease the thickness of theca cell of the female rat model of PCOS


Umayal B, Jayakody S N, Chandrasekharan N V, Wijesundera W S, Wijeyaratne C N.

Polycystic ovary syndrome (PCOS) and kisspeptin – A Sri Lankan study. J Postgrad Med


Homburg R. Polycystic ovary syndrome. Best Practice & Research Clinical Obstetrics &

Gynaecology. 2008;22(2):261-74.

Fauser BC, Tarlatzis BC, Rebar RW, et al. Consensus on women’s health aspects of polycystic

ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus

Workshop Group. Fertility and sterility. 2012;97(1):28-38. e25.

Touyz RM. Molecular and cellular mechanisms in vascular injury in hypertension: role of

angiotensin II–editorial review. Current opinion in nephrology and hypertension.


Rojas J, Chávez M, Olivar L, et al. Polycystic ovary syndrome, insulin resistance, and obesity:

navigating the pathophysiologic labyrinth. International journal of reproductive medicine.


Azziz R, Carmina E, Dewailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Futterweit

W, et al. The androgen excess and PCOS society criteria for the polycystic ovary syndrome:

the complete task force report. Fertility and Sterility. 2009;91(2).

Ma Y, Andrisse S, Chen Y, Childress S, Xue P, Wang Z, et al. Androgen receptor in the ovary

theca cells plays a critical role in androgen-induced reproductive dysfunction.Endocrinology

Jan 1; 158(1): 98–108.

Huang Y, Sun J, Wang X, Tao X, Wang H, Tan W. Asymptomatic chronic gastritis decreases

metformin tolerance in patients with type 2 diabetes. Journal of clinical pharmacy and

therapeutics. 2015;40(4):461-5.

Johnson B. Clinical perspectives on the health effects of Moringa oleifera: A promising

adjunct for balanced nutrition and better health. KOS health publications; 2005.

Adewole SO, Caxton-Martins EA, Ojewole JA. Protective effect of quercetin on the

morphology of pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of

Traditional, Complementary and Alternative Medicines. 2007;4(1):64-74.

Sujatha B, Patel P. Moringa Oleifera–Nature’s Gold. Imperial Journal of Interdisciplinary

Research. 2017;3(5)

Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and

dyslipidemia: a review. Frontiers in pharmacology. 2012;3.

Purwanto B, Sudiana IK, Herawati L, Aksono B. MUSCLE GLUCOSE TRANSPORTER 1



Vendola KA, Zhou J, Adesanya OO, Weil SJ, Bondy CA. Androgens stimulate early stages of

follicular growth in the primate ovary. Journal of Clinical Investigation. 1998;101(12):2622.

Wu C, Lin F, Qiu S, Jiang Z. The characterization of obese polycystic ovary syndrome rat

model suitable for exercise intervention. PloS one. 2014;9(6):e99155

Rzepczynska IJ, Foyouzi N, Piotrowski PC, Celik-Ozenci C, Cress A, Duleba AJ.

Antioxidants induce apoptosis of rat ovarian theca-interstitial cells. Biology of reproduction.


Jahan S, Munir F, Razak S, et al. Ameliorative effects of rutin against metabolic, biochemical

and hormonal disturbances in polycystic ovary syndrome in rats. Journal of ovarian research.





How to Cite

Wulandari, L. P., & Hapsari, A. (2020). The Effect of Moringa oleifera Leaf Extract on Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance. PROCEEDING SURABAYA INTERNATIONAL HEALTH CONFERENCE 2019, 1(1), 298–303. Retrieved from