Cellulase Pretreatment of Kappaphycus alvarezii polymer for Promising Medical Dressing Application


  • Dyah Rika Ar Rosyidah Institut Teknologi Sepuluh Nopember
  • Dini Ermavitalini Institut Teknologi Sepuluh Nopember
  • Maharani Pertiwi Koentjoro Universitas Nahdlatul Ulama Surabaya
  • Isdiantoni Universitas Wiraraja
  • Endry Nugroho Prasetyo Institut Teknologi Sepuluh Nopember


Seaweed contains hydrocolloid polysaccharides which are useful for the application of wound dressing. The use of natural ingredients such as seaweed Kappahycus alvarezii requires pretreatment before it is applied. Pretreatment of seaweed Kappaphycus alvarezii flour was carried out using hydrolyzing enzyme called cellulase. The cellulase (EC is an enzyme which could hydrolyze cellulose polymers thereby breaking β- 1,4 glycosidic bond into oligosaccharide and simple sugars. Meanwhile the cell wall of seaweed could as well be degraded partially by cellulase followed by releasing hydrocolloid polymers (carrageenan) from the inner layer of seaweed cell wall. The aims of this study is to determine the effect of enzymatic pretreatment on seaweed so that it can be useful for further research as one of the promising component in wound dressing. The result showed that the reducing sugar decreased in content of 0.5 mg /ml after enzymatic modification at 0.5 hours incubation time. The FTIR spectra showed shifting at several functional groups namely C-H, C-O-C and C=C.


Aramwit P. 2016. Introduction to biomaterials for wound healing. In Wound Healing

Biomaterials (pp. 3-38).

Mogosanu G D, & Grumezescu A. M. 2014. Natural and synthetic polymers for wounds and

burns dressing. Int J Pharm, 463(2), 127-136. doi:10.1016/j.ijpharm.2013.12.015.

Mahdavinia G R, Rahmani Z, Karami S, & Pourjavadi A. 2014. Magnetic/pH-sensitive kappa-

carrageenan/sodium alginate hydrogel nanocomposite beads: preparation, swelling behavior, and

drug delivery. J Biomater Sci Polym Ed, 25(17), 1891-1906.


Kamoun E A, Kenawy E S, & Chen X. 2017. A review on polymeric hydrogel membranes for

wound dressing applications: PVA-based hydrogel dressings. J Adv Res, 8(3), 217-233.


Sandeep C, Harikumar SL and Kanupriya. 2012. Hydrogels: A Smart Drug Delivery System.

IJRPC 2(3).

Dhivya S, Saravanan S, Sastry T. P, & Selvamurugan N. 2015. Nanohydroxyapatite-reinforced

chitosan composite hydrogel for bone tissue repair in vitro and in vivo. J Nanobiotechnology,

, 40. doi:10.1186/s12951-015-0099-z.

Distantina S, Fadilah F, & Kaavessina M. 2016. Swelling Behaviour of Kappa Carrageenan

Hydrogel in Neutral Salt Solution.

Knudsen N R, Ale M T, Meyer A S. 2015. Seaweed Hydrocolloid Production: An Update on

Enzyme Assisted Extraction and Modification Technologies. Mar. Drugs, 13, 3340-3359;


Abowei J F N, Ezekiel E N. 2013. The potentials and utilization of Seaweeds. Sci. Agri. 4 (2):


Kılınç B, Cirik S, Turan G, Tekogul H., Koru E. 2013. Seaweeds for Food and Industrial

Applications. Intech, Turkey.

Dewi E N, Ibrahim R., Suhartoc S. 2015. Morphological Structure Characteristic And Quality of

Semi Refined Carrageenan Processed By Different Drying Methods. Procedia Environmental

Sciences 23: 116 – 122.

Karaki N, Aljawish A, Humeau C, Muniglia L & Jasniewski J. 2016. Enzymatic modification of

polysaccharides: mechanisms, properties, and potential applications.

Puri M, Sharma D, Barrow C J. 2012. Enzyme-assisted extraction of bioactives from plants.

Trends in Biotechnology, Vol. 30(1).doi:10.1016/j.tibtech.2011. 06.014.

Rabinovich M, Melnik M & Bolobova A. 2002. Microbial cellulases: A review. Appl. Biochem.

Microbiol., 38, 305-321.

Ranveer R C, Patil S N, Sahoo A K. 2013. Effect of different parameters on enzyme-assisted

extractionof lycopene from tomato processing waste. Food and Bioproducts Processing 9(1):


Karima A, Nurhatika S, and Nugroho Prasetyo E. 2016. Enzymatic Modification of Cellulose

Based Materials for Pomising of Bioethanol Production. IJMSE.

Ghose T.K. 1987. Measurement of Cellulase Activities. Pure Appl Chem 59 (2): 257-268.

Bradford M.M. 1967. A Repaid Sensitive Method for the Guanntification of Microgram

Quantities Of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry

, 248-254.

Ahn C B, Jeon Y J, Kang D S, Shin T S, Jung B M. 2004. Free radical scavenging activity of

enzymatic extracts from a brown seaweed Scytosiphon lomentaria by electron spin resonance

spectrometry. Food Research International 37 (2004) 253–258.

Alrumman S A. 2016. Enzymatic Saccharification And Fermentation Of Cellulosic Date Palm

Wastes To Glucose And Lactic Acid. Brazilian journal of microbiology 4 7 (2 0 1 6) 110–119

Sartori T, Tibolla H, Prigol E, Colla L M, Costa J A V, Bertolin T E. 2014. Enzymatic

Saccharification of Lignocellulosic Residues by Cellulases Obtained from Solid State

Fermentation Using Trichoderma viride. BioMed Research International Volume 2015, Article

ID 342716, http://dx.doi.org/10.1155/2015/342716

Miller G L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar.

Analytical Chemistry, vol. 31, no. 3, pp. 426–428.

Foreman P K, Brown D, Dankmeyer L, Dean R, Diener S, Dunn-Coleman N S, Goedegebuur F,

Houfek T D, England GJ, Kelley AS, Meerman HJ, Mitchell T, Mitchinson C, Olivares HA,

Teunissen PJ, Yao J, & Ward M. 2003. Transcriptional Regulation of Biomass Degrading

Enzymes in the Filamentous Fungus Trichoderma reesei. The Journal of Biological Chemistry

Vol.278, No. 34, pp.31988-31997.

Khopkar S M. 1990. Konsep Dasar Kimia Analitik, Terjemahan Basic Concepts of Analytical

Chemistry. Jakarta.: Penerbit Universitas Indonesia.

Foreman PK, Brown D, Dankmeyer L, Dean R, Diener, S., Dunn-Coleman, N.S., Goedegebuur

F, Houfek TD, England GJ, Kelley AS, Meerman HJ, Mitchell T, Mitchinson C, Olivares HA,

Teunissen PJ., Yao J, & Ward M. 2003. Transcriptional Regulation of Biomass Degrading

Enzymes in the Filamentous Fungus Trichoderma reesei. The Journal of Biological Chemistry

Vol.278, No. 34, pp.31988-31997.

Gritzali M. & Brown R D, Jr. 1979. The cellulase system of Trichoderma : relationship between

purified extracellular enzymes from induced and cellulose grown cells. Advances in Chemistry

Series 181, 237-260.

Mackuľak T, Prousek J, Olejníkováa P, Bodík I. 2013. The Using of Enzymes for Degradation

of Cellulose Substrate for The Production Of Biogas. 2010. In Proceedings of the 37th

International Conference of Slovak Society of Chemical Engineering, Tatranské Matliare,

Slovakia, 1407–1412.

Tanaka M, Ikesaka M, Matsuno R, Converse A.O. 1988. Effect of Pore-Size in Substrate and

Diffusion of Enzyme on Hydrolysis of Cellulosic Materials with Cellulases. Biotechnology and

Bioengineering, 32(5), 698-706.

Rojas O J, Jeong C, Turon X, Argyropoulos DS. 2006. Measurement of Cellulase Activity with

Piezoelectric Resonators. Forest Biomaterials Laboratory, College of Natural Resources, NC

State University, Raleigh, NC 27695.

Pereira L. (2013). Population studies and carrageenan properties in eight Gigartinales

(Rhodophyta) from Western Coast of Portugal. ScientificWorldJournal, 2013, 939830.


Vera J, Castro J, Gonzales A, Moenne A. 2011. Seaweed Polysaccharides and Derived

Oligosaccharides Stimulate Defense Responses and Protection Against Pathogens in Plants.

Mar. Drugs (2011), 9, 2514-2525.

Rahnama N, Mamat S, Shah U K M, Ling FH, Rahman NA, Ariff A B. 2013. Effect of Alkali

Pretreatment of Rice Straw on Cellulase and Xylanase Production by Local Trichoderma

harzianum SNRS3 under Solid State Fermentation. Bioresources 8(2): 2881-2896.

Ciolacu D, Cilolacu F, & Popa VI 2011. Amorphous Cellulose–Structure and Characterization.

Cellulose Chem. Technol., 45 (1- 2), 13-21.

Dai D, & Fan M. 2011. Investigation of the Dislocation of Natural Fibres by Fouriertransform

Infrared Spectroscopy. Spectroscopy, Vol.55, No.2, pp. 300-306




How to Cite

Rosyidah, D. . R. . A. . ., Ermavitalini, D. . ., Koentjoro, M. . P. . ., Isdiantoni, & Prasetyo, E. N. (2020). Cellulase Pretreatment of Kappaphycus alvarezii polymer for Promising Medical Dressing Application. PROCEEDING SURABAYA INTERNATIONAL HEALTH CONFERENCE 2019, 1(1), 99–107. Retrieved from https://conferences.unusa.ac.id/index.php/SIHC19/article/view/507