The Effectiveness Extracts Of Ocimum Sanctum L Leaf On Motility And Morphology Spermatozoa Mice That Is Exposure With Cigarette Smoke
Keywords:
Morphology Spermatozoa, Ocimum Sanctum L Leaf, Cigarette SmokeAbstract
The incidence of infertility is still a health problem in the world including Indonesia
(21.3%) throughout Asia. Fertility in men is strongly influenced by several factors, one of
them is smoking behavior. Smoking can increase ROS, reduce antioxidants in semen, cause
DNA damage, and morphological abnormalities of spermatozoa. Basil contains flavonoids
which are rich in antioxidants, boron and zinc to stimulate of androgen hormones. This
research is using 8- weeks-old Mus musculus, we exposed with smoke of 20 cigarettes/day
and we administered of extract Ocimum sanctum L in dose dependency (100 mg/kg,
200mg/kg, and 300mg/kg) for 4 weeks. Then we examined the motility and morphology of
spermatozoa by microscope. This research consists of 5 group. In CS-, CSBL-2 and CSBL-3
it showed that the spermatozoa motility is more progressive than CS+ and CSBL-1. The
morphology of spermatozoa in CS+ and CS- found different results with a significance value
0,10 (p0.05). There is an effect of giving basil leaves to increase motility and improvement
the morphology of spermatozoa that have been exposed to cigarette smoke especially in dose
300 mg/kgBB but not significant
References
Anita, N. (2004). Perubahan Sebaran Stadia Epitel Seminiferus, Penurunan Jumlah Sel-Sel
Spermatogenik dan Kadar Hormon Testosteron Total Mencit (Mus musculus L) Galur DDY Yang Diberi
Asap Rokok Kretek. Skripsi. Jakarta: Universitas Indonesia.
Batubara, Immanuel.V.D., Wantouw, Benny., Tendean, L. (2013). Pengaruh Paparan Asap Rokok
Kretek Terhadap Kulitas Spermatozoa Mencit Jantan (Mus Musculus). Journal eBiomedik (eBM). 1(1).
Christijanti, W., Utami, N.R & Iswara. A. (2010). Efek pemberian antioksidan vitamin C dan e
terhadap kualitas spermatozoa tikus putih terpapar allethrin. Biosaintifika. 2(1): 18–26.
Cummins, J.M., Jequier, A.M & Kan. R. (1994). Molecular biology of human male infertility: Links
with aging, mitochondrial genetics, and oxidative stress?. Mol Reprod Dev. 37(3): 345– 62.
Desi, N. H., Kurniasari, D., Romdhoni, M. F., Maulana, A. M. (2018). Pengaruh Pemberian Ekstrak
Etanol Daun Kemangi (Ocimum Basilicum L.) Terhadap Motilitas Spermatozoa Tikus Putih Galur
Wistar Jantan (Rattus Norvegicus) Yang Diinduksi Monosodium Glutamate (Msg). Jurnal Ilmu
Kesehatan dan Kedokteran Keluarga. 14(1): 48–54.
Faranita OV. (2009). Kualitas spermatozoa pada tikus wistar jantan diabetes melitus. Skripsi.
Semarang: Universitas Diponegoro.
Fitriani, E. K & Sari. W. (2009). The effect of cigarettes smoke exposured causes fertility of male
mice (Mus musculus). Jurnal Natural, 10(2): 1–6.
Gunawan, D & Mulyani, S. (2004) Ilmu Obat Alam, Farmakognosi. I. Jakarta: Penerbit Penebar
Swadaya.
Hafaz, NA. (2017). The effect of fasting to the quality of spermatozoa in adult male rattus
norvegicus. J Kedokt dan Kesehat Indones. 8(2): 2001–2.
Himpunan Endokrinologi Reproduksi dan Fertilitas Indonesia (HIFERI). (2013) Konsensus
Penanganan Infertilitas.
Karim, D. (2011). Pengaruh paparan asap rokok elektrik terhadap motilitas, jumlah sperma dan
kadar mda testis mencit (Mus musculus L.). Skripsi. Medan: Sumatera Utara.
Khaki A, Fathiazad F, Nouri M, K. A. (2011). Effect of Ocimum basilicum on apoptosis in testis of
rats after exposure to electromagnetic field. 5(12): 1534–7.
Kuniawan, S. (2013). Daun Kemangi, Bawang Merah, Bawang Putih dan Bengkuang Terapi
Herbal Kesehatan dan Kecantikan. Yogyakarta: Diva Press.
Mascarenhas. Maya N. et al. (2012). National. Regional. and Global Trends in Infertility
Prevalence Since 1990: A Systematic Analysis of 277 Health Surveys. PLOS Medicine.
Mahendra, T. (2009). Pengaruh Pemberian Ekstrak Rimpang Jahe Merah (Zingiber officinale
roscoe var. rubrum) Terhadap Motilitas dan Konsentrasi Spermatozoa Tikus Putih (Rattus Novergicus)
Jantan. Skripsi. Surabaya: Universitas Airlangga
. [16] Murod, A. M. (2014). Uji Aktivitas Ekstrak Air Herba Kemangi (Ocimum americanum L.)
terhadap Kualitas Sperma dan Densitas Sel Spermatogenesis Tikus Sprague-Dawley Jantan secara in
vivo. Skripsi. Jakarta: Universitas UIN Syarif Hidayatullah.
Philip GH, Dereddy Nagalinga Reddy, Kadiri Pullanna, G. H. and Philip (2015). Effects of Cigarette
Smoking on Semen Parameters of Men. Department of Zoology. India: Sri Krishnadevaraya
University.
Safwan S, Sugara T, R. M. (2016). Pengaruh Ekstrak Daun Kemangi (Ocimum sanctum L.)
Terhadap Motilitas dan Konsentrasi Spermatozoa Mencit Jantan (Mus musculus). J Ilm Ibnu Sina Ilmu
Farm dan Kesehat. 1(2): 173–81.
Sarma D, Sai Koteswar and Babu, A. V. S. (2011). Pharmacognostic and Phytochemical Studies of
Ocimum mericanum. Journal of Chemical and Pharmaceutical Research. 3(3).
Setyo, K. (2013). Obat Ajaib Sirih Merah dan Daun kelor. Yogyakarta: Buku Biru.
Srivastava S., P. Desai, E. Coutinho, G. G. (2006). Mechanism of Action of L-Arginine on the
Vitality of Spermatozoa is Primarily Through Increased Biosynthesis of Nitric Oxide. Biology of
Reproduction Journal. 74: 954–958.
Sukmaningsih A. (2009). Penurunan jumlah spermatosit pakiten dan spermatid tubulus
seminiferus testis mencit (Mus musculus) yang dipaparkan asap rokok. Jurnal Biologi. 12: 31–2.
Sukmaningsih, AASA., Igayum, E., Wiratmini, NI., Sudatri, N. (2011). Gangguan spermatogenesis
setelah pemberian monosodium glutamat pada mencit (mus musculus l.). J Biol. 15(2).
