Bactericidal effects of Extract Bacil Leaves in in-vitro study of Pesudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is the most common bacterial cause of nosocomial infections. Bacteria
become resistant to antibiotics by various mechanisms, including producing enzymes that can damage
antibiotics, change intracellular targets from antibiotics and efflux pumps. Basil (Ocimum sanctum L.) is a
traditional plant that is usually used as ingredients that contain antibacterial compounds including flavonoids,
tannins, alkaloids, eugenol. The aim was to determine the effectiveness of extract basil leaves (Ocimum sanctum
L.) for inhibiting and killing the growth of Pseudomonas aeruginosa. We administrated the extract basil leaves
(Ocimum sanctum L.) with a concentration of 100%, 50%, and 25% in to the plate contained bacterium
Pseudomonas aeruginosa. The result showed that broth dilution for 8 hours and 24 hours there is no inhibition of
bacterial growth. And we continued to cultured bacteria for 24 hours. The analysis showed the extract of
Ocimum sanctum L has bactericidal effects in 8 hours and 24 hours incubation significantly (p <0.05). However,
in 24 hours more effective as a bactericidal in 100% of concentration significantly (p <0.05). From this result,
eugenol which is a phenol derivative found in the ethanol extract of basil leaves has the effect of damaging cell
membranes. Phenol bonding with bacterial cell walls can disrupt the permeability of transport cell membranes so
that the bacteria will be disrupted and die.The extract of basil leaves effective as a bactericidal to Pseudomonas
aeruginosa